Regulation of Gene Expression

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Structural Studies Of DNA and RNA Transactions

Our studies of nucleic acid transactions have been most powerful at defining the overall architecture of a large number of macromolecular assemblies involved in the critical initiation steps in DNA replication, RNA transcription and translation, for which no crystallographic information yet exist (e.g. ORC, TFIID, eIF3), as well as putting the crystal structures of essential elements in RNA processing and degradation in the complex of fully functional complexes (e.g. exosome, RLC). Our aim is to gain mechanistic insight that goes beyond overall architecture, by pushing resolution, describing conformational landscapes, and relating structural states to function via the analysis of interactions with ligands and regulatory factors.