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Xu, C.S., Hayworth, K.J., Lu, Z., Grob, P.,Hassan, A., Garcia Cerdan, J.G., Niyogi, K.K., Nogales, E., Weinberg, R.J. and Hess, H.F. Enhanced FIB-SEM systems for large-volume 3D imaging eLife doi: 10.7554/eLife.25916.