Molecular Degradation

Proteasome-mediated proteolysis

Eukaryotic cells contains thousands of different proteins, many of which are required for only brief periods during the cellular cycle. These short-lived proteins, along with any misfolded or damaged intracellular proteins, are destroyed by a large proteolytic complex named the 26S proteasome.... read more


CRISPR-mediated prokaryotic immunity

In E. coli, CRISPR transcripts are processed into short RNAs that are incorporated into a large ribonucleoprotein surveillance complex called Cascade. This complex specifically targets and binds foreign nucleic acids in a sequence-specific manner, followed by degradation of the targeted substrate. We used cryo-electron microscopy to determine the structure of Cascade before and after binding to a target substrate... read more


Exosome and RNA Processing

The eukaryotic exosome is a conserved complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the nine-subunit core exosome and provides the sole sources of processive 3'-to-5' exoribonuclease activity. We have obtained EM reconstructions of the core and Rrp44-bound exosome complexes... read more



Recent publications

Kellogg et al. <a href=http://www.sciencedirect.com/science/article/pii/S0022283617300153\> Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures</a><i> JCB</i>, 2017 Mar 10; Epub ahead of print Yuan et al. <a href=http://www.nature.com/nature/journal/v533/n7603/full/nature17970.html\> Near-atomic resolution visualization of human transcription promoter opening</a><i> Nature</i>, 2016 May 11; Epub ahead of print Louder et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/27007846> Structure of promoter-bound TFIID and model of human pre-initiation complex assembly</a><i> Nature</i>, 2016 Mar 23;531(7596):604-9 Jiang and Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/26841432> Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.</a> <i>Science</i>, Jan 2016; Epub ahead of print Nogales and Zhang<a href=http://www.sciencedirect.com/science/article/pii/S0959440X16000038> Visualizing microtubule structural transitions and interactions with associated proteins</a> <i>COSB</i>, April 2016; <b>37</b>, 90-96  Zhang et al. <a href=http://www.sciencedirect.com/science/article/pii/S0092867415008491> Mechanistic origin of microtubule dynamic instability and its modulation by EB proteins</a><i> Cell 2015 Aug 13;162(4):849-59

Eva Nogales has been elected to the American Academy of Arts and Sciences

Basil Greber and Kelly Nguyen have won the 2016 RNA Society Scaringe Award

Eva Nogales has won the 2016 Mildred Cohn Award in biological chemistry from the American Society for Biochemistry and Molecular Biology

Harvey Lecture by Eva Nogales