Molecular Degradation

Proteasome-mediated proteolysis

Eukaryotic cells contains thousands of different proteins, many of which are required for only brief periods during the cellular cycle. These short-lived proteins, along with any misfolded or damaged intracellular proteins, are destroyed by a large proteolytic complex named the 26S proteasome.... read more


CRISPR-mediated prokaryotic immunity

In E. coli, CRISPR transcripts are processed into short RNAs that are incorporated into a large ribonucleoprotein surveillance complex called Cascade. This complex specifically targets and binds foreign nucleic acids in a sequence-specific manner, followed by degradation of the targeted substrate. We used cryo-electron microscopy to determine the structure of Cascade before and after binding to a target substrate... read more


Exosome and RNA Processing

The eukaryotic exosome is a conserved complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the nine-subunit core exosome and provides the sole sources of processive 3'-to-5' exoribonuclease activity. We have obtained EM reconstructions of the core and Rrp44-bound exosome complexes... read more



Recent publications

The following publications have been accepted and are currently in press. Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/25837515> Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.</a><i>Science</i>, 2015 Apr 2, Epub ahead of print Baskaran et al. <a href=http://elifesciences.org/content/early/2014/12/09/eLife.05115> Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex</a><i>eLife</i>, 2014;10.7554 Epub Raymond Staals, Yifan Zhu, David Taylor et al. <a href=http://www.sciencedirect.com/science/article/pii/S1097276514007898> RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus.</a> <i>Mol Cell</i> <b>56</b>, 518-539 Greg Alushin et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24855948> High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis</a><i>Cell</i>, 157(5)1117-29. Megan Hochstrasser, David Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24748111> CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.</a><i>Proc Natl Acad Sci</i>, <b>111(18)</b>, 6618-23.

Jack Kornfeld received the Molecular and Cell Biology Departmental Citation, the highest honor given to a graduating senior in MCB.

Eva Nogales has been elected to the prestigious National Academy of Sciences.

Eva Nogales was announced as the receipient of the 2015 Dorothy Crowfoot Hodgkin Award. This award is sponsored by Genentech, and granted in recognition of exceptional contributions in protein science which profoundly influence our understanding of biology.