Molecular Degradation

Proteasome-mediated proteolysis

Eukaryotic cells contains thousands of different proteins, many of which are required for only brief periods during the cellular cycle. These short-lived proteins, along with any misfolded or damaged intracellular proteins, are destroyed by a large proteolytic complex named the 26S proteasome.... read more


CRISPR-mediated prokaryotic immunity

In E. coli, CRISPR transcripts are processed into short RNAs that are incorporated into a large ribonucleoprotein surveillance complex called Cascade. This complex specifically targets and binds foreign nucleic acids in a sequence-specific manner, followed by degradation of the targeted substrate. We used cryo-electron microscopy to determine the structure of Cascade before and after binding to a target substrate... read more


Exosome and RNA Processing

The eukaryotic exosome is a conserved complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the nine-subunit core exosome and provides the sole sources of processive 3'-to-5' exoribonuclease activity. We have obtained EM reconstructions of the core and Rrp44-bound exosome complexes... read more



Recent publications

The following publications have been accepted and are currently in press. Martin Jinek et al. <a href=http://www.sciencemag.org/content/early/2014/02/05/science.1247997> Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation</a><i>Science</i>, Epub ahead of print Bleichert et al. <a href=http://elife.elifesciences.org/content/2/e00882> A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation</a><i>eLife</i>, 2:e00882, Oct 8 2013 Epub Kassube et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/23748380> Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5</a><i>Nat. Struct. Mol. Biol.</i>, 20(7)892-9 Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/23624860> Substrate-specific structural rearrangements of human Dicer.</a><i>Nat. Struct. Mol. Biol.</i>, 20(6)662-70

Forbes: 30 Under 30 Issue

Alumni Greg Alushin (class of 2012, Biophysics Graduate Program) is one of the 30 under 30 selected by the magazine Forbes in the category of Science and Healthcare.