Structural Studies Of DNA and RNA Transactions

Our studies of nucleic acid transactions have been most powerful at defining the overall architecture of a large number of macromolecular assemblies involved in the critical initiation steps in DNA replication, RNA transcription and translation, for which no crystallographic information yet exist (e.g. ORC, TFIID, eIF3), as well as putting the crystal structures of essential elements in RNA processing and degradation in the complex of fully functional complexes (e.g. exosome, RLC). Our aim is to gain mechanistic insight that goes beyond overall architecture, by pushing resolution, describing conformational landscapes, and relating structural states to function via the analysis of interactions with ligands and regulatory factors.

Eukaryotic DNA Replication

During cell division, initiator proteins target and bind to DNA replication origins to trigger genome duplication. We are characterizing the structure of Drosophila melanogaster Origin Recognition Complex (DmORC), and the effect of nucleotide and phosphorylation state in conformation and function ... read more about ORC ...


Eukaryotic Transcription

Regulated gene transcription in eukaryotes requires the assembly of a complex molecular machinery that includes general factors, activators, cofactor complexes and chromatin modifying and remodeling factors. We are interested in characterizing the structure of these different components and how they interact to regulate transcription. Read more about chromatin remodelers ...   Read more about TFIID ...   Read more about RNAPII...   Read more about PRC2...


Eukaryotic Translation Initiation

In eukaryotes at least twelve translation initiation factors and an mRNA methylated G-cap are required to initiate protein synthesis, while in many viruses a structured RNA element replaces most of those factors. We are studying the molecular interplays that characterize both initiation pathways ... read more about eIF3


Transcription-Coupled DNA Repair

Our lab is part of a large NCI funded project in Structural Biology of DNA Repair that aims at producing biologically relevant DNA repair structures and identifying fundamental structural principles for repair proteins. The role of electron microscopy is to provide structures of large macromolecular complexes involved in these processes... read more


Recent publications

The following publications have been accepted and are currently in press. Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/25837515> Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.</a><i>Science</i>, 2015 Apr 2, Epub ahead of print Baskaran et al. <a href=http://elifesciences.org/content/early/2014/12/09/eLife.05115> Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex</a><i>eLife</i>, 2014;10.7554 Epub Raymond Staals, Yifan Zhu, David Taylor et al. <a href=http://www.sciencedirect.com/science/article/pii/S1097276514007898> RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus.</a> <i>Mol Cell</i> <b>56</b>, 518-539 Greg Alushin et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24855948> High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis</a><i>Cell</i>, 157(5)1117-29. Megan Hochstrasser, David Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24748111> CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.</a><i>Proc Natl Acad Sci</i>, <b>111(18)</b>, 6618-23.

Jack Kornfeld received the Molecular and Cell Biology Departmental Citation, the highest honor given to a graduating senior in MCB.

Eva Nogales has been elected to the prestigious National Academy of Sciences.

Eva Nogales was announced as the receipient of the 2015 Dorothy Crowfoot Hodgkin Award. This award is sponsored by Genentech, and granted in recognition of exceptional contributions in protein science which profoundly influence our understanding of biology.