HHMI UCB LBL

Eukaryotic Cytoskeleton In Cell Division

Cell division is a highly regulated process requiring the interplay of physical and chemical cues. The cytoskeleton is a central player, serving as energy source for dramatic movements requiring complex mechano-chemistry, as well as acting as a scaffold that facilitates molecular encounters at the right time and place within the cell. We study two cytoskeletal systems, microtubules (MTs) and septin filaments, that play critical roles in mitosis and cytokinesis, respectively. Importantly, their self-assembly and interactions can serve both as input or readout in checkpoint processes that regulate critical and often irreversible steps in cell division progression.

Structural Basis of Microtubule Dynamic Instability

In our microtubule cytoskeleton studies we are interested in defining the conformational landscape of tubulin as defined by its nucleotide and assembly states, in order to obtain detail mechanistic understanding of the process of microtubule dynamic instability ... read more

microtubules

Microtubule-Kinetochore Interface

In the cell the dynamics of microtubules are regulated and made use of by their interaction with different factors. Of special interest is the coupling of microtubules to the kinetochore, a process where microtubule dynamics reaches its "climax". We pursue a mechanistic understanding of the molecular interactions governing the regulated, dynamic attachment of kinetochores to microtubules that underlies the accurate segregation of chromosomes during mitosis ... read more

Dam1

Septins Structure And Assembly

Septins are conserved GTases defining a new type of cytoskeletal filaments essential in cytokinesis and other membrane-remodeling processes. We are studying the yeast septins to define the organization of different septin assembly units, their polymerization and their regulation ... read more

Filament