Electron Microscopy of Macromolecular Assemblies

The Nogales's lab is dedicated to gaining mechanistic insight into two important areas of eukaryotic biology: central dogma machinery in the control of gene expression, and cytoskeleton interactions and dynamics in cell division. The unifying principle in our work is the study of macromolecular assemblies as whole units of molecular function by direct visualization of their architecture, functional states, and regulatory interactions. To gain a molecular understanding of their systems of interest, our lab uses state-of-the-art cryo-electron microscopy (cryo-EM) and image analysis, as well as biochemical and biophysical assays.


Recent publications

Liu JJ et al. <a href=https://www.nature.com/articles/s41586-019-0908-x> CasX enzymes comprise a distinct family of RNA-guided genome editors </a> Nature 2019 Feb 04 Patel et al. <a href=http://science.sciencemag.org/content/early/2018/11/14/science.aau8872> Structure of human TFIID and mechanism of TBP loading onto promoter DNA </a> Science 2018 Nov 15 Kellogg et al. <a href=http://science.sciencemag.org/content/early/2018/05/09/science.aat1780?rss=1> Near-atomic model of microtubule-tau interactions </a> Science 2018 May 10 Nguyen et al. <a href= https://www.nature.com/articles/s41586-018-0062-x>  Cryo-EM structure of substrate-bound human telomerase holoenzyme.
</a> Nature 2018 Apr 25 Poepsel et al. <a href=https://www.nature.com/articles/s41594-018-0023-y > Cryo-EM structures of PRC2 simultaneously engaged with two functionally distinct nucleosomes. </a> Nature Structural and Molecular Biology 2018 Jan 29 Kasinath et al. <a href=http://science.sciencemag.org/content/early/2018/01/17/science.aar5700 > Structures of human PRC2 with its cofactors AEBP2 and JARID2. </a> Science 2018 Jan 18

Lab member, Basil Greber , will be an ICR Fellow, in the Division of Structural Biology at the Institute of Cancer Research, London, UK

Lab Memeber, Vignesh Kasinath , will be starting as an Assistant Professor in the Department of Biochemistry at CU Boulder

Eva Nogales Named a 2020 Biophysical Society Fellow EMBO

Lab Member, Vignesh Kasinath has been awarded the the NIH Pathway to Independence (K99) award

Lab Member, Kelly Nguyen , will be starting as a Group Leader at the MRC-Laboratory of Molecular Biology in Cambridge, UK in August 2019

Lab member, Simon Poepsel, has started an Independent Research Group at the Center for Molecular Medicine at the University of Cologne, Germany in June 2019