HHMI UCB LBL

Electron Microscopy of Macromolecular Assemblies

The Nogales's lab is dedicated to gaining mechanistic insight into two important areas of eukaryotic biology: central dogma machinery in the control of gene expression, and cytoskeleton interactions and dynamics in cell division. The unifying principle in our work is the study of macromolecular assemblies as whole units of molecular function by direct visualization of their architecture, functional states, and regulatory interactions. To gain a molecular understanding of their systems of interest, our lab uses state-of-the-art cryo-electron microscopy (cryo-EM) and image analysis, as well as biochemical and biophysical assays.

News

Recent publications

Kellogg et al. <a href=http://www.sciencedirect.com/science/article/pii/S0022283617300153\> Insights into the Distinct Mechanisms of Action of Taxane and Non-Taxane Microtubule Stabilizers from Cryo-EM Structures</a><i> JCB</i>, 2017 Mar 10; Epub ahead of print Yuan et al. <a href=http://www.nature.com/nature/journal/v533/n7603/full/nature17970.html\> Near-atomic resolution visualization of human transcription promoter opening</a><i> Nature</i>, 2016 May 11; Epub ahead of print Louder et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/27007846> Structure of promoter-bound TFIID and model of human pre-initiation complex assembly</a><i> Nature</i>, 2016 Mar 23;531(7596):604-9 Jiang and Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/26841432> Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.</a> <i>Science</i>, Jan 2016; Epub ahead of print Nogales and Zhang<a href=http://www.sciencedirect.com/science/article/pii/S0959440X16000038> Visualizing microtubule structural transitions and interactions with associated proteins</a> <i>COSB</i>, April 2016; <b>37</b>, 90-96  Zhang et al. <a href=http://www.sciencedirect.com/science/article/pii/S0092867415008491> Mechanistic origin of microtubule dynamic instability and its modulation by EB proteins</a><i> Cell 2015 Aug 13;162(4):849-59

Eva Nogales has been elected to the American Academy of Arts and Sciences

Basil Greber and Kelly Nguyen have won the 2016 RNA Society Scaringe Award

Eva Nogales has won the 2016 Mildred Cohn Award in biological chemistry from the American Society for Biochemistry and Molecular Biology

Harvey Lecture by Eva Nogales