The Nogales’s lab is dedicated to gaining mechanistic insight into two important areas of eukaryotic biology: central dogma machinery in the control of gene expression, and cytoskeleton interactions and dynamics in cell division. The unifying principle in our work is the study of macromolecular assemblies as whole units of molecular function by direct visualization of their architecture, functional states, and regulatory interactions. To gain a molecular understanding of their systems of interest, our lab uses state-of-the-art cryo-electron microscopy (cryo-EM) and image analysis, as well as biochemical and biophysical assays.