2010

Nogales, E., Ramey, V.H. and Wang, H-W. (2010) Cryo-EM studies of structural intermediates in microtubule dynamic instability and interaction of microtubules with kinetochore complexes. In "Microtubule Methods in vitro". Edt. Les Wilson and John Correia. Elsevier, in press.

2009

Nogales, E. and Ramey, V.H. (2009) Structure-function insights into the yeast Dam1 kinetochore complex. J Cell Sci., 22, 3831-3836.

Wang, H.-W., Noland, C., Siredichadilok, B., Taylor, D.W., Ma, E., Felderer, K., Doudna, J.A., Nogales, E. (2009) Structural insights into RNA Processing by the Human RISC-Loading Complex. Nat Struct Mol Biol., 16, 1148-1153.

Wu, Z., Wang, H-W., Mu, W., Ouyang, Z., Nogales, E., Xing, J. (2009) Simulations of tubulin sheet polymers as possible structural intermediates in microtubule assembly. PLoS One, 4(10): e7291.

Liu, W-L., Coleman, R.A., Yang, J-L., Grob, P., Ma, E., Dailey, G., Nogales, E., and Tjian, R. (2009) Structures of three distinct activator-TFIID complexes. Genes Dev., 23(13), 1510-1521.

Ramey, V.H., Wang, H.-W. and Nogales, E. (2009) Ab initio reconstruction of helical samples with heterogeneity, disorder and coexisting symmetries. J Struct Biol., 167(2), 97-105.

Wang, H.-W., Ramey, V.H., Westermann, S., Drubin, D., Barnes, G., and Nogales, E. (2009) The yeast Dam1 kinetochore complex (also known as DASH) is essential for proper chromosome segregation... HFSP J., 3(2), cover only.

Scheres, S.H., Valle, M., Grob, P., Nogales, E., and Carazo, J.M. (2009) Maximum likelihood refinement of electron microscopy data with normalization errors. J Struct Biol., 166(2), 234-240.

2008

Nogales, E. and Downing, K.H. (2008) Tubulin and Microtubule Structures. The Role of Microtubules in Cell Biology, Neurobiology, and Oncology, T. Fojo (Ed.), Humana Press.

Wang, H.-W., Long, S., Ciferri, C., Westermann, S., Drubin, D., Barnes, G. and Nogales, E. (2008) Architecture and Flexibility of the Yeast Ndc80 Kinetochore Complex. J Mol Biol., 383(4), 894-903.

Clarey, M.G., Botchan, M. and Nogales, E. (2008) Single particle EM studies of the Drosophila melanogaster origin recognition complex and evidence for DNA wrapping. J Struct Biol., 164(3), 241-249.

Bertin, A., McMurray, M.A., Grob, P., Park, S.S., Garcia III, G., Patanwala, I., Ng, H.L., Alber, T., Thorner, J. and Nogales, E. (2008) Saccharomyces cerevisiae septins: supramolecular organization of heterooligomers and the mechanism of filament assembly. Proc Natl Acad Sci., 105(24), 8274-8279.

Liu, W-L., Coleman, R.A., Grob, P., Geles, K.G., King, D.S., Ramey, V.H., Nogales, E. and Tjian, R. (2008) Structural changes in TAF4b-TFIID correlated with promoter selectivity. Mol Cell., 29, 81-91.

2007

Wang, H.-W., Wang, J., Ding, F., Callahan, K., Bulter, J.S., Nogales, E., and Ke, A. (2007) Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3'-end processing. Proc Natl Acad Sci., 104, 16844-16849.

Wang, H.-W., Ramey, V.H., Westermann, S., Leschziner, A., Welburn, J.P.I., Nakajima, Y., Drubin, D.G., Barnes, G., and Nogales, E. (2007) Architecture of the Dam1 kinetochore ring complex: implications for microtubule-driven assembly and force-coupling mechanisms. Nat Struct Mol Biol., 14, 721-726.

Hall, R.L., Siridechadilok, B., and Nogales, E. (2007) Cross-correlation of common lines: a novel approach for single-particle reconstruction of a structure containing a flexible domain. J Struct Biol., 159, 474-482.

Khodiyar, V.K., Maltais, L.J., Sneddon, K.M.B., Smith, J., Shimoyama, M., Cabral, F., Dumontet, C., Dutcher, S.K., Lafanechère, L., Murray, J.W., Nogales, E., Piquemal, D., Stanchi, F., Povey, S., and Lovering, R.C. (2007) A revised nomenclature for the human and rodent α-tubulin gene family. Genomics, 90, 285-289.

Garczarek, F., Dong, M., Typke, D., Witkowska, E., Hazen, T., Nogales, E., Biggin, M.D., and Glaeser, R.M. (2007) Octomeric pyruvate-ferredoxin oxidoreductase from desulfovibrio vulgaris. J Struct Biol., 159, 9-18

Nogales, E. and Sundquist, W.I. (2007) Macromolecular assemblages: focusing on complexity. Curr Opin Struct Biol., 17, 205-208.

E. Galburt, S.W. Grill, A. Wiedmann, M. Kireeva, E. Nogales, M. Kashlev, and C. Bustamante. (2007) Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner. Nature, 446, 820-823.

A.E. Leschziner, A. Saha, J. Wittmeyer, Y. Zhang, C. Bustamante, B.R. Cairns, and E. Nogales. (2007) Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method. Proc Natl Acad Sci., 104, 4913-4918.

B. Chen, M. Doucleff, D.E. Wemmer, S. DeCarlo, H. Huang, E. Nogales, T.R. Hoover, E. Elena Kondrashkina, L. Guo, and B.T. Nixon. (2007) ATP ground- and transition states of the enhancer binding AAA+ ATPases support complex formation with their target protein, σ54. Structure, 15, 429-440.

A.E. Leschziner and E. Nogales. (2007) Visualizing flexibility at molecular resolution: analysis of heterogeneity in single-particle electron microscopy reconstructions. Ann Rev Biophys Biomol Struct., 36, 43-62.

2006

S.A. Kostek, P. Grob, S. De Carlo, J.S. Lipscomb, F. Garczarek and E. Nogales. Molecular architecture and conformational flexibility of human RNA polymerase II. (2006) Structure, 14, 1691-1700.

M.G. Clarey, J.P. Erzberger, P. Grob, A.E. Leschziner, J.M. Berger, E. Nogales and M. Botchan. Nucleotide-dependent conformational changes in the DnaA-like core of the origin recognition complex. (2006) Nat Struct Mol Biol. 13, 684-690

S. De Carlo, B. Chen, T.R. Hoover, E. Kondrashkina, E. Nogales and B.T. Nixon. The structural basis of regulated assembly and function of the transcriptional activator NtrC. (2006) Genes Dev. 20, 1485-1495.

E. Nogales and H.-W. Wang. Structural mechanisms underlying nucleotide-dependent self assembly of tubulin and its relatives. (2006) Curr Opin Struct Biol. 16(2), 221-229.

H.-W. Wang and E. Nogales. Structural intermediates in microtubule assembly and disassembly: how and why? (2006) Curr Opin Cell Biol. 18(2), 179-284.

P. Grob, M.J. Cruse, C. Inoue, M. Peris, P.A. Penczek, R. Tjian and E. Nogales. Cryo-electron microscopy studies of human TFIID: Conformational breathing in the integration of gene regulatory cues. (2006) Structure. 14, 511-520.

A.E. Leschziner and E. Nogales. The orthogonal tilt reconstruction method: An approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles. (2006) J Struct Biol. 153, 284-299.

S. Westermann, H.-W. Wang, A. Avila-Sakar, D.G. Drubin, E. Nogales and G. Barnes. The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. (2006) Nature. 440, 565-569.

2005

B. Siridechadilok, C.S. Fraser, R.J. Hall, J.A. Doudna, and E. Nogales. Structural roles for human translation factor eIF3 in the initiation of protein synthesis. (2005) Science. 310, 1513-1515.

A.H. Sarker, S.E. Tsutakawa, S. Kostek, C. Ng, D.S. Shin, M. Peris, E. Campeau, J.A. Tainer, E. Nogales and P.K. Cooper. Recognition of RNA Pol II and transcription bubbles by XPG, CSB and TFIIH: Insights for transcription-coupled repair and Cockayne Syndrome. (2005) Mol Cell. 20, 1-12.

Hong-Wei Wang, S.B.T. Long, K.R. Finley, Eva Nogales. Assembly of helical ribbons by GMPCPP-bound tubulin and effect of colchicine. (2005) Cell Cycle. 4, 1157-60.

Hong-Wei Wang and Eva Nogales. The nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. (2005) Nature. 435, 911-915.

Andres E. Leschziner, Bryan Lemon, Robert Tjian, Eva Nogales. Structural Studies of the Human PBAF Chromatin-Remodeling Complex. (2005) Structure. 13, 267-275.

Hong-Wei Wang and Eva Nogales. An iterative Fourier-Bessel algorithm for reconstruction of helical structures with severe Bessel overlap. (2005) J Struct Biol. 149, 65-78.

Stefan Westermann, Agustin Avila-Sakar, Hong-Wei Wang, Hanspeter Niederstrasser, Jonathan Wong, David G. Drubin, Eva Nogales, and Georjana Barnes. Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex. (2005) Mol Cell. 17, 277-290.

2004

E. Nogales. Tubulin and its isoforms. in Encyclopedia of Biological Chemistry, Vol. 4 (2004) W. Lennarz and D. Lane (Eds.), Elsevier Science.

M.S. Poruchynsky, J.H Kim, E. Nogales, T. Annable, F. Loganzo, L.M. Greenberger, D.L. Sackett, T. Fojo. Tumor cells resistant to a microtubule-depolymerizing hemiasterlin analogue, HTI-286, have mutations in α- or β-tubulin and increased microtubule stability. (2004) Biochemistry. 43, 13944-13954.

2003

Eva Nogales, Hong-Wei Wang, and Hanspeter Niederstrasser. Tubulin Rings: Which Way Do They Curve? (2003) Curr Opin Struct Biol. 13, 256-261. (review)

2002

Hanspeter Niederstrasser, Hani Salehi-Had, Eugene C. Gan, Claire Walczak, Eva Nogales. XKCM1 Acts on a Single Protofilament and Requires the C Terminus of Tubulin. (2002) J Mol Biol. 316(3), 817-828.

Dylan J. Taatjes, Anders M. Näär, Frank Andel III, Eva Nogales, Robert Tjian. Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator. (2002) Science. 295, 1058-1062.

Heald R., Nogales E. Microtubule dynamics. (2002) J Cell Sci. 115, 3-4.

A.M. Näär, D.J. Taatjes, W. Zhai, E. Nogales, and R. Tjian. Human CRSP interacts with RNA polymerase II CTD and adpots a specific CTD-bound conformation. (2002) Genes Dev., 16, 1339-1344.

H. Li, D.J. DeRosier, W.V. Nicholson, E. Nogales, and K.H. Downing. Microtubule structure at 8 Å resolution. (2002) Structure, 10, 1317-1328.

2001

J. Löwe, H. Li, K.H. Downing, E. Nogales. (2001) Refined Structure of αβ-Tubulin at 3.5 Å Resolution. J Mol Biol. 313, 1045-1057.

Agustin J. Avila-Sakar, Shahram Misaghi, Elizabeth Wilson-Kubalek, Kenneth H. Downing, Helen Zgurskaya, Hiroshi Nikaido and Eva Nogales. (2001) Lipid-Layer Crystallization and Preliminary Three-Dimensional Structural Analysis of AcrA, the Periplasmic Component of a Bacterial Multidrug Efflux Pump. J Struct Biol. 136, 81-88.

M. Katherine Jung, Natalie Prigozhina, C. Elizabeth Oakley, Eva Nogales, and Berl R. Oakley. (2001) Alanine-scanning Mutagenesis of Aspergillus γ-Tubulin Yields Diverse and Novel Phenotypes. Mol Biol Cell. 12(7), 2119-36.

E. Nogales. (2001) Structural insight into microtubule function. Annu Rev Biophys Biomol Struct. 30, 397-420.

Snyder J.P., Nettles J.H., Cornett B., Downing K.H., Nogales E. (2001) The binding conformation of Taxol in β-tubulin: A model based on electron crystallographic density. Proc Natl Acad Sci USA. 98(9), 5312-5316.

Eva Nogales, N. Grigorieff. (2001) Molecular Machines: Putting the Pieces Together. J Cell Biol. 152, F1-10. (review)

Y.F. Inclan, E. Nogales. (2001) Structural models for the self-assembly and microtubule interactions of gamma-, delta- and epsilon-tubulin. J Cell Sci. 114(2), 413-422.

2000

H. William Detrich III, Sandra K. Parker, Robley C. Williams Jr., Eva Nogales, Kenneth H. Downing. Cold Adaptation of Microtubule Assembly and Dynamics. J Biol Chem. 275(47), 37038-37047, 2000.

P. Giannakakou, R. Gussio, Eva Nogales, Kenneth H. Downing, D. Zaharevitz, B. Bollbuck, G. Poy, D. Sackett, K.C. Nicolaou, T. Fojo. A common pharmacophore for epothilone and taxanes: Molecular basis for drug resistance conferred by tubulin mutations in human cancer cells. Proc Natl Acad Sci USA. 97(6), 2904-9, 2000.

J.L. Paluh, Eva Nogales, B.R. Oakley, K. McDonald, A.L. Pidoux, W.Z. Cande. A Mutation in γ-Tubulin Alters Microtubule Dynamics and Organization and Is Synthetically Lethal with the Kinesin-like Protein Pkl1p. Mol Cell Biol. 11, 1225-39, 2000.

K.L. Richards, K.R. Anders, Eva Nogales, K. Schwartz, K.H. Downing, D. Botstein. Structure-Function Relationships in Yeast Tubulins. Mol Cell Biol. 11, 1887-903, 2000.

Eva Nogales. Recent structural insights into transcription preinitiation complexes. J Cell Sci. Pt 24, 4391-7, 2000.

1999

Y. Han, E.P. Sablin, E. Nogales, R.J. Fletterick, K.H. Downing. Visualizing a New Binding Site of ncd-Motor Domain on Tubulin. J Struct Biol. 128(1), 26-33, 1999.

Eva Nogales, Wah Chiu. Electron Crystallography of Biological Macromolecules (Editorial). J Struct Biol. 128(1), 1-2, 1999.

Eva Nogales, M Whittaker, RA Milligan, Kenneth H. Downing. High-resolution model of the microtubule. Cell. 96(1), 79-88, 1999.

Becket Feierbach, Eva Nogales, Kenneth H. Downing, Tim Stearns. Alf1p, a CLIP-170 Domain-containing Protein, Is Functionally and Physically Associated with α-Tubulin. J Cell Biol. 144, 113-24, 1999.

WV Nicholson, M Lee, Kenneth H. Downing, Eva Nogales. Cryo-electron microscopy of GDP-tubulin rings. Cell Biochem Biophys. 2, 175-83, 1999.

Eva Nogales. A structural view of microtubule dynamics. Cell Mol Life Sci. 56(1-2), 133-42, 1999.

Frank Andel III, AG Ladurner, C Inouye, R Tjian, Eva Nogales. Three-dimensional structure of the human TFIID-IIA-IIB complex. Science. 286, 2153-6, 1999.

1998

Eva Nogales, Sharon G. Wolf, Kenneth H. Downing. Structure of the αβ-tubulin dimer by electron crystallography. Nature. 391, 199-203, 1998.

Kenneth H. Downing, Eva Nogales. Tubulin and microtubule structure. Curr Opin Cell Biol. 10, 16-22, 1998.

Eva Nogales, Kenneth H. Downing, Linda A Amos, Jan Löwe. Tubulin and FtsZ form a distinct family of GTPases. Nat Struct Biol. 6, 451-8, 1998.

Kenneth H. Downing, Eva Nogales. Tubulin structure: insights into microtubule properties and functions. Curr Opin Struct Biol. 8, 785-791, 1998.

Summaries

A Historical Overview of Tubulin and Microtubule Structure

Reviews and Profiles

Picturing Proteins. Berkeley Lab Research Review, Winter 2000 issue.

Scientists Snap First 3-D Pictures of the "Heart" of the Transcription Machine. UC Berkeley Campus News, December 9, 1999.

Structure of Human Transcription Factor Proteins Revealed In 3D. Berkeley Lab Research News, December 9, 1999.

Did you ever wonder about the skeletal structure of living cells? Berkeley Lab, 1999.

Mystery of Vital Cell Protein Solved After 30 Years. Berkeley Lab Research News, January 8, 1998.