HHMI UCB LBL

Animations

This QuickTime movie represents a comparison of Structural Models for the Transcrition Preinitiation Complex (PIC).

The animation is available for download in 1024x388 and 1920x728 resolutions.

Structural Models for the Transcription Preinitiation Complex (PIC)

Cryo-EM reconstruction of purified yeast PIC with available crystal structures docked from Murikami, Kornberg and colleagues.
Murikami et al. Science DOI:10.1126/science 1238724 (2013)

Negative stain reconstruction of step-wise assembled humen PIC with available crystal structures docked from He, Nogales and colleagues
He et al. Nature 495, 481-486 (2013)

Cryo-EM reconstruction of partial human PIC (lacking TFIIH) with available crystal structures docked from He, Nogales and colleagues.
He et al. Nature 495, 481-486 (2013)

Microtubules - Structure, Function and Dynamics

This QuickTime movie, representing the work of Eva Nogales and Hong-Wei Wang, was created by Stylus Visualization Studio.

The movie is currently available at 400x300 resolution, either with or without narration. The narrated version also contains additional footage in the form of a brief introduction.

no sound

nogales_400x300.mov (70 MB)
400 x 300 resolution, no sound
(requires QuickTime)

narrated

nogales_400x300_narrated.mov (93 MB)
400 x 300 resolution, narrated
(requires QuickTime)

News

Recent publications

Greg Alushin et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24855948> High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis</a><i>Cell</i>, 157(5)1117-29. Megan Hochstrasser, David Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24748111> CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference.</a><i>Proc Natl Acad Sci</i>, Epub ahead of print Martin Jinek et al. <a href=http://www.sciencemag.org/content/early/2014/02/05/science.1247997> Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation</a><i>Science</i>, Epub ahead of print Bleichert et al. <a href=http://elife.elifesciences.org/content/2/e00882> A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation</a><i>eLife</i>, 2:e00882, Oct 8 2013 Epub Kassube et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/23748380> Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5</a><i>Nat. Struct. Mol. Biol.</i>, 20(7)892-9

Thesis award

David Taylor received the Mary Ellen Jones Dissertation Prize, which is awarded annually to recognize the most distinguished dissertation in Molecular Biophysics and Biochemistry at Yale University.

Forbes: 30 Under 30 Issue

Alumni Greg Alushin (class of 2012, Biophysics Graduate Program) is one of the 30 under 30 selected by the magazine Forbes in the category of Science and Healthcare.