This QuickTime movie represents a comparison of Structural Models for the Transcrition Preinitiation Complex (PIC).

The animation is available for download in 1024x388 and 1920x728 resolutions.

Structural Models for the Transcription Preinitiation Complex (PIC)

Cryo-EM reconstruction of purified yeast PIC with available crystal structures docked from Murikami, Kornberg and colleagues.
Murikami et al. Science DOI:10.1126/science 1238724 (2013)

Negative stain reconstruction of step-wise assembled humen PIC with available crystal structures docked from He, Nogales and colleagues
He et al. Nature 495, 481-486 (2013)

Cryo-EM reconstruction of partial human PIC (lacking TFIIH) with available crystal structures docked from He, Nogales and colleagues.
He et al. Nature 495, 481-486 (2013)

Microtubules - Structure, Function and Dynamics

This QuickTime movie, representing the work of Eva Nogales and Hong-Wei Wang, was created by Stylus Visualization Studio.

The movie is currently available at 400x300 resolution, either with or without narration. The narrated version also contains additional footage in the form of a brief introduction.

no sound

nogales_400x300.mov (70 MB)
400 x 300 resolution, no sound
(requires QuickTime)


nogales_400x300_narrated.mov (93 MB)
400 x 300 resolution, narrated
(requires QuickTime)


Recent publications

The following publications have been accepted and are currently in press. Nogales and Scheres <a href=http://www.ncbi.nlm.nih.gov/pubmed/26000851> Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity.</a><i>Mol Cell</i>, May 21;58(4):677-689 Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/25837515> Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.</a><i>Science</i>, 2015 May 1;348(6234):581-5 Baskaran et al. <a href=http://elifesciences.org/content/early/2014/12/09/eLife.05115> Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex</a><i>eLife</i>, 2014;10.7554 Epub Raymond Staals, Yifan Zhu, David Taylor et al. <a href=http://www.sciencedirect.com/science/article/pii/S1097276514007898> RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus.</a> <i>Mol Cell</i> <b>56</b>, 518-539 Greg Alushin et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24855948> High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis</a><i>Cell</i>, 157(5)1117-29.

Read the recent Interview with Eva Nogales featured in the Journal of Cell Biology.
Eva Nogales: See how they run

Vignesh Kasinath received the Saul A. Winegrad Outstanding Dissertation Award from UPenn. The award is given to one person in every department every year for the best dissertation. He received the award for the Biochemistry and Molecular Biophysics Department of which he was a graduate student in.

Jack Kornfeld received the Molecular and Cell Biology Departmental Citation, the highest honor given to a graduating senior in MCB.

Eva Nogales has been elected to the prestigious National Academy of Sciences.

Eva Nogales was announced as the receipient of the 2015 Dorothy Crowfoot Hodgkin Award. This award is sponsored by Genentech, and granted in recognition of exceptional contributions in protein science which profoundly influence our understanding of biology.