This QuickTime movie represents a comparison of Structural Models for the Transcrition Preinitiation Complex (PIC).

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Structural Models for the Transcription Preinitiation Complex (PIC)

Cryo-EM reconstruction of purified yeast PIC with available crystal structures docked from Murikami, Kornberg and colleagues.
Murikami et al. Science DOI:10.1126/science 1238724 (2013)

Negative stain reconstruction of step-wise assembled humen PIC with available crystal structures docked from He, Nogales and colleagues
He et al. Nature 495, 481-486 (2013)

Cryo-EM reconstruction of partial human PIC (lacking TFIIH) with available crystal structures docked from He, Nogales and colleagues.
He et al. Nature 495, 481-486 (2013)

Microtubules - Structure, Function and Dynamics

This QuickTime movie, representing the work of Eva Nogales and Hong-Wei Wang, was created by Stylus Visualization Studio.

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Recent publications

Zhang et al. <a href=http://www.sciencedirect.com/science/article/pii/S0092867415008491> Mechanistic origin of microtubule dynamic instability and its modulation by EB proteins</a><i> Cell 2015 Aug 13;162(4):849-59 Nogales and Scheres <a href=http://www.ncbi.nlm.nih.gov/pubmed/26000851> Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity.</a><i>Mol Cell</i>, May 21;58(4):677-689 Taylor et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/25837515> Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.</a><i>Science</i>, 2015 May 1;348(6234):581-5 Baskaran et al. <a href=http://elifesciences.org/content/early/2014/12/09/eLife.05115> Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex</a><i>eLife</i>, 2014;10.7554 Epub Raymond Staals, Yifan Zhu, David Taylor et al. <a href=http://www.sciencedirect.com/science/article/pii/S1097276514007898> RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus.</a> <i>Mol Cell</i> <b>56</b>, 518-539 Greg Alushin et al. <a href=http://www.ncbi.nlm.nih.gov/pubmed/24855948> High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis</a><i>Cell</i>, 157(5)1117-29.

David Taylor has won an Outstanding Postdoctoral Fellow Award from the Department of Molecular and Cell Biology

Eva Nogales has won the 2016 Mildred Cohn Award in biological chemistry from the American Society for Biochemistry and Molecular Biology

Eva Nogales has been elected to the prestigious National Academy of Sciences.